NSJ Bioreagents

Recombinant Human Nuclear Antigen Antibody

Product Code:
 
NSJ-V9618SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
235-1R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Frozen Section (IHC-F)
Storage:
 
Aliquot the recombinant Human Nuclear Antigen antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Immunofluorescent staining of MeOH-fixed human HeLa cells with recombinant Human Nuclear Antigen antibody (green, clone 235-1R).
2 / 3
Immunofluorescent staining of PFA-fixed human HeLa cells recombinant Human Nuclear Antigen antibody (green, clone 235-1R).
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant Human Nuclear Antigen antibody (clone 235-1R) as confirmation of integrity and purity.
❮❯

Immunofluorescent staining of MeOH-fixed human HeLa cells with recombinant Human Nuclear Antigen antibody (green, clone 235-1R).
Immunofluorescent staining of PFA-fixed human HeLa cells recombinant Human Nuclear Antigen antibody (green, clone 235-1R).
SDS-PAGE analysis of purified, BSA-free recombinant Human Nuclear Antigen antibody (clone 235-1R) as confirmation of integrity and purity.

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NSJ-V9618SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunocytochemistry: 1-2ug/ml,Immunohistochemistry (Frozen): 1-2ug/ml
Description:
This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb235-1R recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for the recombinant Human Nuclear Antigen antibody.
Localisation:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Not Known