NSJ Bioreagents

Transglutaminase 2 Antibody (FN Binding Domain)

Product Code:
 
NSJ-V9619SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
TGM2/3612
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the Transglutaminase 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 2
IHC staining of FFPE human placental tissue with Transglutaminase 2 antibody (clone TGM2/3612). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 2
SDS-PAGE analysis of purified, BSA-free Transglutaminase 2 antibody (clone TGM2/3612) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human placental tissue with Transglutaminase 2 antibody (clone TGM2/3612). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Transglutaminase 2 antibody (clone TGM2/3612) as confirmation of integrity and purity.

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NSJ-V9619SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Tissue transglutaminase (tTG) is a 75 kDa monomeric globular protein expressed in the majority of cells and tissues. tTG localizes mainly in the cytoplasm but some tGT is present on the cell surface and is association with the extracellular matrix. In addition to its guanosine triphosphatase (GTPase) activity, tTG catalyzes the posttranslational modification of proteins by transamidation of available glutamine residues. Extracellular tTG is able to bind to and cross-link several ECM proteins including FN/Fibronectin and SPP1, and may function to stabilize the ECM and basement membranes. The protein is translated as a fully active enzyme and there is no evidence for proteolytic activation. High constitutive expression and accumulation of active tTG is observed in (among others) endothelial cells, platelets, vascular smooth muscle cells and epithelial cells of the lens. tTG is expressed at very low levels in skeletal muscle cells and neurons and may be difficult to detect in these cell/tissue types.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human TGM2 protein was used as the immunogen for the Transglutaminase 2 antibody.
Localisation:
Cytoplasm, Cell surface
Purity:
Protein A/G affinity
Uniprot #:
P21980