NSJ Bioreagents

HIC2 Antibody / Hypermethylated in cancer 2 protein

Product Code:
 
NSJ-V9644
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-HIC2-1B1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
 
Aliquot the HIC2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using HIC2 antibody (green, clone PCRP-HIC2-1B1) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with HIC2 antibody (blue, clone PCRP-HIC2-1B1) and isotype control (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free HIC2 antibody (clone PCRP-HIC2-1B1) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using HIC2 antibody (clone PCRP-HIC2-1B1). These results demonstrate the foremost specificity of the PCRP-HIC2-1B1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Immunofluorescent staining of PFA-fixed human HeLa cells using HIC2 antibody (green, clone PCRP-HIC2-1B1) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with HIC2 antibody (blue, clone PCRP-HIC2-1B1) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free HIC2 antibody (clone PCRP-HIC2-1B1) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using HIC2 antibody (clone PCRP-HIC2-1B1). These results demonstrate the foremost specificity of the PCRP-HIC2-1B1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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CodeSizePrice
NSJ-V9644-20UG20 ug£264.00
Quantity:
NSJ-V9644-100UG100 ug£534.00
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Further Information

Application Details:
ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Description:
HIC2 (HIC ZBTB Transcriptional Repressor 2) is a Protein Coding gene. Diseases associated with HIC2 include Orofaciodigital Syndrome X and Simpson-Golabi-Behmel Syndrome, Type 1. HIC2 contains 5 C2H2-type zinc fingers and 1 BTB (POZ) domain. It belongs to the krueppel C2H2-type zinc-finger protein family, Hic subfamily and is a transcriptional repressor. It is a transcription activator of SIRT1.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human HIC2protein was used as the immunogen for the HIC2 antibody.
Purity:
Protein A/G affinity
Uniprot #:
Q96JB3