NSJ Bioreagents

CNOT10 Antibody / CCR4-NOT transcription complex subunit 10

Product Code:
 
NSJ-V9648
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2c
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-CNOT10-1D5
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Aliquot the CNOT10 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using CNOT10 antibody (green, clone PCRP-CNOT10-1D5) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with CNOT10 antibody (blue, clone PCRP-CNOT10-1D5) and isotype control (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free CNOT10 antibody (clone PCRP-CNOT10-1D5) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CNOT10 antibody (clone PCRP-CNOT10-1D5). These results demonstrate the foremost specificity of the PCRP-CNOT10-1D5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Immunofluorescent staining of PFA-fixed human HeLa cells using CNOT10 antibody (green, clone PCRP-CNOT10-1D5) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with CNOT10 antibody (blue, clone PCRP-CNOT10-1D5) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free CNOT10 antibody (clone PCRP-CNOT10-1D5) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CNOT10 antibody (clone PCRP-CNOT10-1D5). These results demonstrate the foremost specificity of the PCRP-CNOT10-1D5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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NSJ-V9648-20UG20 ug£264.00
Quantity:
NSJ-V9648-100UG100 ug£534.00
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Further Information

Application Details:
ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Description:
CNOT10 is a subunit of the CCR4-NOT complex which consists of at least five other CNOT subunit proteins and TAB182. The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription, as well as in mRNA degradation. Various subunits (e.g. CNOT1, CNOT3) are uniquely involved in influencing nuclear hormone receptor activities. In effect, this complex has an important role as a transcription regulator and repressor of nuclear receptor signaling that is relevant to the molecular pathways involved in cancer. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human protein was used as the immunogen for the CNOT10 antibody.
Localisation:
Cytoplasm (Mitochondrion) and Nucleus (PML body)
Purity:
Protein A/G affinity
Uniprot #:
Q9H9A5