NSJ Bioreagents

GTF2A1 Antibody / TFIIA

Product Code:
 
NSJ-V9649SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-GTF2A1-1F2
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
 
Aliquot the GTF2A1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using GTF2A1 antibody (green, clone PCRP-GTF2A1-1F2) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with GTF2A1 antibody (blue, clone PCRP-GTF2A1-1F2) and isotype control (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free GTF2A1 antibody (clone PCRP-GTF2A1-1F2) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using GTF2A1 antibody (clone PCRP-GTF2A1-1F2). These results demonstrate the foremost specificity of the PCRP-GTF2A1-1F2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Immunofluorescent staining of PFA-fixed human HeLa cells using GTF2A1 antibody (green, clone PCRP-GTF2A1-1F2) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with GTF2A1 antibody (blue, clone PCRP-GTF2A1-1F2) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free GTF2A1 antibody (clone PCRP-GTF2A1-1F2) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using GTF2A1 antibody (clone PCRP-GTF2A1-1F2). These results demonstrate the foremost specificity of the PCRP-GTF2A1-1F2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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NSJ-V9649SAF-100UG100 ug£534.00
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Further Information

Application Details:
ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Description:
Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in formation of the preinitiation complex (PIC) which contains several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. Recognition of the TATA binding element by TBP may be regulated by TFIIA. TFIIA consists of three subunits designated TFIIA-, all of which associate with both TBP and TAF (TBP-associated factor). TFIIA functions to stabilize the interaction between TFIID and DNA by binding directly to TBP and the DNA (at the TATA box), thus forming a TBP/TFIIA/TATA complex which mediates the transcriptional output of a gene.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human protein was used as the immunogen for the GTF2A1 antibody.
Localisation:
Nucleus, cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P52655