NSJ Bioreagents

TFIIB Antibody / GTF2B

Product Code:
 
NSJ-V9650SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-GTF2B-1D1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
Storage:
 
Aliquot the TFIIB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using TFIIB antibody (clone PCRP-GTF2B-1D1). These results demonstrate the foremost specificity of the PCRP-GTF2B-1D1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 3
FACS staining of PFA-fixed human HeLa cells with TFIIB antibody (blue, clone PCRP-GTF2B-1D1) and isotype control (red).
3 / 3
SDS-PAGE analysis of purified, BSA-free TFIIB antibody (clone PCRP-GTF2B-1D1) as confirmation of integrity and purity.
❮❯

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using TFIIB antibody (clone PCRP-GTF2B-1D1). These results demonstrate the foremost specificity of the PCRP-GTF2B-1D1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
FACS staining of PFA-fixed human HeLa cells with TFIIB antibody (blue, clone PCRP-GTF2B-1D1) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free TFIIB antibody (clone PCRP-GTF2B-1D1) as confirmation of integrity and purity.

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NSJ-V9650SAF-100UG100 ug£534.00
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This product comes from: United States.
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Further Information

Application Details:
ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells
Description:
In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Template commitment is established by the initial binding of TFIID to the TATA element of the promotor, a step which may be facilitated by TFIIA. TFIIB then acts as the bridge between TFIID and RNA polymerase II.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human protein was used as the immunogen for the TFIIB antibody.
Localisation:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q00403