NSJ Bioreagents

ZNF81 Antibody

Product Code:
 
NSJ-V9655SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-ZNF81-2G2
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Aliquot the ZNF81 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using ZNF81 antibody (green, clone PCRP-ZNF81-2G2) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with ZNF81 antibody (blue, clone PCRP-ZNF81-2G2) and isotype control (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free ZNF81 antibody (clone PCRP-ZNF81-2G2) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZNF81 antibody (clone PCRP-ZNF81-2G2). These results demonstrate the foremost specificity of the PCRP-ZNF81-2G2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Immunofluorescent staining of PFA-fixed human HeLa cells using ZNF81 antibody (green, clone PCRP-ZNF81-2G2) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with ZNF81 antibody (blue, clone PCRP-ZNF81-2G2) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free ZNF81 antibody (clone PCRP-ZNF81-2G2) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZNF81 antibody (clone PCRP-ZNF81-2G2). These results demonstrate the foremost specificity of the PCRP-ZNF81-2G2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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NSJ-V9655SAF-100UG100 ug£534.00
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Further Information

Application Details:
ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Description:
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a krueppel-type DNAbinding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZNF81, also known as HFZ20 or MRX45, is a transcriptional regulator belonging to the krueppel C2H2-type zincfinger protein family. It localizes to the nucleus and contains 12 C2H2-type zinc fingers and one KRAB domain. Mutations in the gene encoding ZNF81 are implicated in nonsyndromic X-linked mental retardation (XLMR).
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human ZNF81 protein was used as the immunogen for the ZNF81 antibody.
Localisation:
Nucleus, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P51508