NSJ Bioreagents

MIF Antibody / Macrophage Migration Inhibitory Factor

Product Code:
 
NSJ-V9665
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MIF/3490
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the MIF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Western blot testing of human 1) LNCaP and 2) PC3 cell lysates using MIF antibody (clone MIF/3489). Predicted molecular weight ~13 kDa.
2 / 3
IHC staining of FFPE human prostate tissue with MIF antibody (clone MIF/3490). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MIF antibody (clone MIF/3490). These results demonstrate the foremost specificity of the MIF/3490 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Western blot testing of human 1) LNCaP and 2) PC3 cell lysates using MIF antibody (clone MIF/3489). Predicted molecular weight ~13 kDa.
IHC staining of FFPE human prostate tissue with MIF antibody (clone MIF/3490). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MIF antibody (clone MIF/3490). These results demonstrate the foremost specificity of the MIF/3490 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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NSJ-V9665-20UG20 ug£264.00
Quantity:
NSJ-V9665-100UG100 ug£534.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Macrophage migration inhibitory factor, known as MIF or glycosylation-inhibiting factor (GIF), is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection. MIF is expressed at sites of inflammation, which suggests that it plays a role in regulating macrophage function in host defense. MIF is produced by the pituitary gland and is found in monocytes, macrophages, differentiating immunological cells in the eye lens and brain, and fibroblasts. Elevated levels of MIF protein are detected in the plasma of patients with severe sepsis or septic shock, a condition where MIF influences endotoxic shock by enhancing the production of other inflammatory cytokines including tumor necrosis factor). MIF promotes the systemic inflammatory response by counter-regulating glucocorticoid-mediated inhibition of immune-cell activation and proinflammatory cytokine production. MIF may mediate tissue destruction through the induction of proteinases.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant fragment from the human protein was used as the immunogen for the MIF antibody.
Localisation:
Secreted, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P14174