NSJ Bioreagents

Lysozyme C Antibody / LYZ

Product Code:
 
NSJ-V9673
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
LYZ/3944
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Lysozyme C antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 5
Western blot testing of human spleen tissue and HepG2 cell lysates using Lysozyme C antibody (clone LYZ/3944). Predicted molecular weight ~17 kDa.
2 / 5
IHC staining of FFPE human spleen tissue with Lysozyme C antibody (clone LYZ/3944). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human spleen tissue with Lysozyme C antibody (clone LYZ/3944). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
SDS-PAGE analysis of purified, BSA-free Lysozyme C antibody (clone LYZ/3944) as confirmation of integrity and purity.
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Lysozyme C antibody (clone LYZ/3944). These results demonstrate the foremost specificity of the LYZ/3944 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Western blot testing of human spleen tissue and HepG2 cell lysates using Lysozyme C antibody (clone LYZ/3944). Predicted molecular weight ~17 kDa.
IHC staining of FFPE human spleen tissue with Lysozyme C antibody (clone LYZ/3944). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with Lysozyme C antibody (clone LYZ/3944). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Lysozyme C antibody (clone LYZ/3944) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Lysozyme C antibody (clone LYZ/3944). These results demonstrate the foremost specificity of the LYZ/3944 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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NSJ-V9673-20UG20 ug£264.00
Quantity:
NSJ-V9673-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Lysozyme is an enzyme, commonly referred to as the body s own antibiotic since it kills bacteria. Natural substrate of lysozyme is the bacterial cell wall peptidoglycan (cleaving the beta[1-4] glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Lysozyme is synthesized predominantly in reactive histiocytes rather than in resting, unstimulated phagocytes. This antibody labels myeloid cells, histiocytes, granulocytes, macrophages and monocytes. It is helpful in the identification of myeloid or monocytic nature of acute leukemia.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 18-147 was used as the immunogen for the Lysozyme C antibody.
Localisation:
Secreted
Purity:
Protein A/G affinity
Uniprot #:
P61626