NSJ Bioreagents

PI3K Antibody (p85 beta) / PIK3R2

Product Code:
 
NSJ-V9682
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PIK3R2/292
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the PI3K antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 3
IHC staining of FFPE human bladder carcinoma tissue with PIK3 antibody (clone PIK3R2/292). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding.
2 / 3
IHC staining of FFPE human spleen tissue with PIK3 antibody (clone PIK3R2/292). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PI3K antibody (clone PIK3R2/292). These results demonstrate the foremost specificity of the PIK3R2/292 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

IHC staining of FFPE human bladder carcinoma tissue with PIK3 antibody (clone PIK3R2/292). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding.
IHC staining of FFPE human spleen tissue with PIK3 antibody (clone PIK3R2/292). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PI3K antibody (clone PIK3R2/292). These results demonstrate the foremost specificity of the PIK3R2/292 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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CodeSizePrice
NSJ-V9682-20UG20 ug£264.00
Quantity:
NSJ-V9682-100UG100 ug£534.00
Quantity:
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85 heterotrimeric G proteins).
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 520-720 was used as the immunogen for the PI3K antibody.
Localisation:
Cytoplasm, Nucleus
Purity:
Protein A/G affinity
Uniprot #:
O00459