NSJ Bioreagents

NFIA Antibody / Nuclear Factor 1 A

Product Code:
 
NSJ-V9686
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2c
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-NFIA-2C6
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the NFIA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 6
IHC staining of FFPE human lung tissue with NFIA antibody (clone PCRP-NFIA-2C6). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human colon carcinoma tissue with NFIA antibody (clone PCRP-NFIA-2C6). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 6
FACS staining of PFA-fixed human HeLa cells with NFIA antibody (blue, clone PCRP-NFIA-2C6) and isotype control (red).
4 / 6
Immunofluorescent staining of PFA-fixed human HeLa cells using NFIA antibody (green, clone PCRP-NFIA-2C6) and phalloidin (red).
5 / 6
SDS-PAGE analysis of purified, BSA-free NFIA antibody (clone PCRP-NFIA-2C6) as confirmation of integrity and purity.
6 / 6
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using NFIA antibody (clone PCRP-NFIA-2C6). These results demonstrate the foremost specificity of the PCRP-NFIA-2C6 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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IHC staining of FFPE human lung tissue with NFIA antibody (clone PCRP-NFIA-2C6). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma tissue with NFIA antibody (clone PCRP-NFIA-2C6). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
FACS staining of PFA-fixed human HeLa cells with NFIA antibody (blue, clone PCRP-NFIA-2C6) and isotype control (red).
Immunofluorescent staining of PFA-fixed human HeLa cells using NFIA antibody (green, clone PCRP-NFIA-2C6) and phalloidin (red).
SDS-PAGE analysis of purified, BSA-free NFIA antibody (clone PCRP-NFIA-2C6) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using NFIA antibody (clone PCRP-NFIA-2C6). These results demonstrate the foremost specificity of the PCRP-NFIA-2C6 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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NSJ-V9686-20UG20 ug£264.00
Quantity:
NSJ-V9686-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Nuclear factor (NF) proteins are a family of CCAAT-box-binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA-binding domain, and activates transcription through a putatively novel, proline-rich, carboxy-terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, -globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant protein fragment was used as the immunogen for the NFIA antibody.
Localisation:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q12857