NSJ Bioreagents

MEF2D Antibody

Product Code:
 
NSJ-V9704SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-MEF2D-3A4
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the MEF2D antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 5
Immunofluorescent staining of PFA-fixed human HeLa cells using MEF2D antibody (green, clone PCRP-MEF2D-3A4) and phalloidin (red).
2 / 5
FACS staining of PFA-fixed human HeLa cells with MEF2D antibody (blue, clone PCRP-MEF2D-3A4) and isotype control (red).
3 / 5
IHC staining of FFPE human rhabdomyosarcoma tissue with MEF2D antibody (clone PCRP-MEF2D-3A4). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
SDS-PAGE analysis of purified, BSA-free MEF2D antibody (clone PCRP-MEF2D-3A4) as confirmation of integrity and purity.
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MEF2D antibody (clone PCRP-MEF2D-3A4). These results demonstrate the foremost specificity of the PCRP-MEF2D-3A4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Immunofluorescent staining of PFA-fixed human HeLa cells using MEF2D antibody (green, clone PCRP-MEF2D-3A4) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with MEF2D antibody (blue, clone PCRP-MEF2D-3A4) and isotype control (red).
IHC staining of FFPE human rhabdomyosarcoma tissue with MEF2D antibody (clone PCRP-MEF2D-3A4). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free MEF2D antibody (clone PCRP-MEF2D-3A4) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MEF2D antibody (clone PCRP-MEF2D-3A4). These results demonstrate the foremost specificity of the PCRP-MEF2D-3A4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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NSJ-V9704SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Description:
The myocyte enhancer factor-2 (MEF-2) family of transcription factors associate with co-repessors or co-activators to regulate development and function of T cells, neuronal cells and muscle cells. Four family members arise from alternatively spliced transcripts, termed MEF-2A, -2B, -2C and -2D. These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes. Differential regulation in the expression of the four transcripts implies functional distinction for each duing embryogenesis and development. The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including MyoD, myogenin, Myf-5 and MRF4, are one class of identified factors. A second family of DNA-binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF-2) family. Each of these proteins binds to the MEF-2 target DNA sequence present in the regulatory regions of many muscle-specific genes.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human MEF2D protein was used as the immunogen for the MEF2D antibody.
Localisation:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q14814