NSJ Bioreagents

L-Myc Antibody / MYCL1

Product Code:
 
NSJ-V9705
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-MYCL-2D5
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Aliquot the L-Myc antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using L-Myc antibody (clone PCRP-MYCL-2D5). These results demonstrate the foremost specificity of the PCRP-MYCL-2D5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using L-Myc antibody (green, clone PCRP-MYCL-2D5) and phalloidin (red).
3 / 4
FACS staining of PFA-fixed human HeLa cells with L-Myc antibody (blue, clone PCRP-MYCL-2D5) and isotype control (red).
4 / 4
SDS-PAGE analysis of purified, BSA-free L-Myc antibody (clone PCRP-MYCL-2D5) as confirmation of integrity and purity.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using L-Myc antibody (clone PCRP-MYCL-2D5). These results demonstrate the foremost specificity of the PCRP-MYCL-2D5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Immunofluorescent staining of PFA-fixed human HeLa cells using L-Myc antibody (green, clone PCRP-MYCL-2D5) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with L-Myc antibody (blue, clone PCRP-MYCL-2D5) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free L-Myc antibody (clone PCRP-MYCL-2D5) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V9705-20UG20 ug£264.00
Quantity:
NSJ-V9705-100UG100 ug£534.00
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This product comes from: United States.
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Further Information

Application Details:
ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Description:
Oncogene-encoded proteins c-Myc, N-Myc, and L-Myc function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors, the N-Myc gene in neuroblastomas, and the L-Myc gene in human small cell lung carcinomas. c-Myc protein is a transcription factor localized to the nucleus of the cell. It seems to be involved in activating the transcription of growth-related genes. c-Myc binds to DNA during transcription as a heterodimeric complex with Max. c-Myc is phosphorylated in vitro by p44/42 MAP kinase at Ser62 and in vivo at both Thr58 and Ser62. Mutation of Thr58 and Ser62 to Ala inhibits the ability of c-Myc to activate transcription.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human MYCL protein was used as the immunogen for the L-Myc antibody.
Localisation:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
P12524