NSJ Bioreagents

PBX2 Antibody

Product Code:
 
NSJ-V9707
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-PBX2-1C4
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
 
Aliquot the PBX2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 3
FACS staining of PFA-fixed human HeLa cells with PBX2 antibody (blue, clone PCRP-PBX2-1C4) and isotype control (red).
2 / 3
SDS-PAGE analysis of purified, BSA-free PBX2 antibody (clone PCRP-PBX2-1C4) as confirmation of integrity and purity.
3 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PBX2 antibody (clone PCRP-PBX2-1C4). These results demonstrate the foremost specificity of the PCRP-PBX2-1C4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

FACS staining of PFA-fixed human HeLa cells with PBX2 antibody (blue, clone PCRP-PBX2-1C4) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free PBX2 antibody (clone PCRP-PBX2-1C4) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PBX2 antibody (clone PCRP-PBX2-1C4). These results demonstrate the foremost specificity of the PCRP-PBX2-1C4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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NSJ-V9707-20UG20 ug£264.00
Quantity:
NSJ-V9707-100UG100 ug£534.00
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Further Information

Application Details:
ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Description:
Pbx 1, 2, 3 and 4 are members of the TALE (three amino acid loop extension) family of homeodomain-containing proteins. Human pre-B cell acute leukemias are frequently associated with a t(1;19)(q23;p13.3) chromosomal rearrangement, which creates a chimeric gene encoding a fusion between the E2A and Pbx 1 gene products. Pbx 2 and Pbx 3 share 92% and 94% respective identities with Pbx 1 over a 266 amino acid region flanking their homeobox domains, while all three proteins are quite divergent at their amino- and carboxy-termini. Two forms of Pbx 1 and Pbx 3 each differ primarily in their carboxy-termini and result from alternative mRNA splicing. Unlike other homeotic selector genes which are expressed transiently during development and differentiation, Pbx gene transcripts are ubiquitously expressed in both fetal and adult tissues and cell lines. Additionally, Pbx 2 and Pbx 3 transcripts are detected in lymphoid cells, which do not express Pbx 1. Pbx 4 expression is confined to the testis, especially to spermatocytes in the pachytene stage of the first meiotic prophase.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human PBX2 protein was used as the immunogen for the PBX2 antibody.
Localisation:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
P40425