NSJ Bioreagents

BCL11A Antibody / B-cell lymphoma/leukemia 11A

Product Code:
 
NSJ-V9710
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-BCL11A-1G10
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Aliquot the BCL11A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using BCL11A antibody (green, clone PCRP-BCL11A-1G10) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with BCL11A antibody (blue, clone PCRP-BCL11A-1G10) and isotype control (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free BCL11A antibody (clone PCRP-BCL11A-1G10) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using BCL11A antibody (clone PCRP-BCL11A-1G10). These results demonstrate the foremost specificity of the PCRP-BCL11A-1G10 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Immunofluorescent staining of PFA-fixed human HeLa cells using BCL11A antibody (green, clone PCRP-BCL11A-1G10) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with BCL11A antibody (blue, clone PCRP-BCL11A-1G10) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free BCL11A antibody (clone PCRP-BCL11A-1G10) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using BCL11A antibody (clone PCRP-BCL11A-1G10). These results demonstrate the foremost specificity of the PCRP-BCL11A-1G10 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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CodeSizePrice
NSJ-V9710-20UG20 ug£264.00
Quantity:
NSJ-V9710-100UG100 ug£534.00
Quantity:
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Further Information

Application Details:
ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Description:
Bcl-11a (CtIP-1, EVI9, B cell chronic lymphocytic leukemia (CLL)/lymphoma 11A) and Bcl-11b (CtIP-2, RIT1, B cell CLL/lymphoma 11B) genes play crucial roles in lymphopoiesis and influence the progression of hematopoietic malignancies. Disruption of the Bcl-11b locus is linked to T cell acute lymphoblastic leukemia, and the loss of heterozygosity in mice results in T cell lymphoma. Bcl-11 proteins are related C2H2 zinc-finger transcription factors that act as transcriptional repressors. Bcl-11b can interact with the metastasis-associated proteins MTA1 and MTA2 through the amino-terminal region. Bcl-11a is essential for postnatal development and normal lymphopoiesis. The Bcl-11a mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with Bcl-6.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human protein was used as the immunogen for the BCL11A antibody.
Localisation:
Cytoplasm, Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q9H165