NSJ Bioreagents

PIK3R2 / PI3K Antibody (p85 beta)

Product Code:
 
NSJ-V9712SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PIK3R2/1293
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the PIK3R2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 3
IHC staining of FFPE human bladder tissue with PIK3R2 antibody (clone PIK3R2/1293). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human bladder tissue with PIK3R2 antibody (clone PIK3R2/1293). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PIK3R2 antibody (clone PIK3R2/1293). These results demonstrate the foremost specificity of the PIK3R2/1293 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC staining of FFPE human bladder tissue with PIK3R2 antibody (clone PIK3R2/1293). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder tissue with PIK3R2 antibody (clone PIK3R2/1293). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PIK3R2 antibody (clone PIK3R2/1293). These results demonstrate the foremost specificity of the PIK3R2/1293 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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NSJ-V9712SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85 heterotrimeric G proteins.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 520-720 was used as the immunogen for the PIK3R2 antibody.
Localisation:
Cytoplasm, Nucleus
Purity:
Protein A/G affinity
Uniprot #:
O00459