NSJ Bioreagents

PRMT7 Antibody

Product Code:
 
NSJ-V9719
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-PRMT7-1A7
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Aliquot the PRMT7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using PRMT7 antibody (green, clone PCRP-PRMT7-1A7) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with PRMT7 antibody (blue, clone PCRP-PRMT7-1A7), and unstained cells (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free PRMT7 antibody (clone PCRP-PRMT7-1A7) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PRMT7 antibody (clone PCRP-PRMT7-1A7). These results demonstrate the foremost specificity of the PCRP-PRMT7-1A7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Immunofluorescent staining of PFA-fixed human HeLa cells using PRMT7 antibody (green, clone PCRP-PRMT7-1A7) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with PRMT7 antibody (blue, clone PCRP-PRMT7-1A7), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free PRMT7 antibody (clone PCRP-PRMT7-1A7) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PRMT7 antibody (clone PCRP-PRMT7-1A7). These results demonstrate the foremost specificity of the PCRP-PRMT7-1A7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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CodeSizePrice
NSJ-V9719-20UG20 ug£264.00
Quantity:
NSJ-V9719-100UG100 ug£534.00
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Further Information

Application Details:
Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells
Description:
Arginine methylation is an irreversible protein modification catalyzed by Arginine methyltransferases, such as PRMT7, which uses S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport and RNA splicing. PRMT7 has two methyltransferase domains, each containing a putative AdoMet-binding motif. The N-terminal methyltransferase domain closely resembles the catalytic core of PRMT5, and the C-terminal domain is most similar to that of PRMT1. Three PRMT7 splice variants have been identified by database analysis. PRMT7 is localized to the nucleus and cytoplasm and moderate expression is observed in adult brain and lung tissues.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human PRMT7 protein was used as the immunogen for the PRMT7 antibody.
Localisation:
Nucleus, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
Q9NVM4