NSJ Bioreagents

Recombinant Prostein Antibody / SLC45A3

Product Code:
 
NSJ-V9764
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SLC45A3/7176R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant Prostein antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant Prostein antibody (clone SLC45A3/7176R). These results demonstrate the foremost specificity of the SLC45A3/7176R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 3
IHC staining of FFPE human prostate tissue with recombinant Prostein antibody (clone SLC45A3/7176R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant Prostein antibody (clone SLC45A3/7176R) as confirmation of integrity and purity.
❮❯

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant Prostein antibody (clone SLC45A3/7176R). These results demonstrate the foremost specificity of the SLC45A3/7176R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
IHC staining of FFPE human prostate tissue with recombinant Prostein antibody (clone SLC45A3/7176R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Prostein antibody (clone SLC45A3/7176R) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V9764-20UG20 ug£264.00
Quantity:
NSJ-V9764-100UG100 ug£534.00
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This product comes from: United States.
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Human prostein is a 553 aa protein identified by cDNA library subtraction and subsequent high throughput microarray screening of prostate cancer. Prostein has multiple transmembrane domains. Prostein has been shown to be uniquely expressed in normal and cancerous prostatic tissues. By immunohistochemistry, prostein is expressed in the vast majority of normal and malignant prostatic tissues, regardless of grade and metastatic status. No protein expression is detected in normal and malignant tissue samples representing the great majority of essential tissues and tumors. In particular, prostein is expressed in most of poorly differentiated prostatic carcinoma, including small cell prostate carcinoma. Prostein is more specific and sensitive for prostatic carcinomas than PSA and PSAP.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A synthesized peptide corresponding to the N terminus of the human protein was used as the immunogen for the recombinant Prostein antibody.
Localisation:
Membrane, Vesicles, Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q96JT2