Further Information
ScienCell's GeneQuery™ Human Antiviral Innate Immune Response qPCR Array Kit (GQH-AII) profiles 88 genes involved in the human antiviral innate immune response. Upon viral infection, most of the cells detects viral pathogen-associated molecular patterns (PAMPs), such as doublestranded RNA, through pattern recognition receptors (PRRs) like Toll-like receptors (TLRs). This recognition triggers several signal transduction pathways, culminating in the production of interferons. Interferons play a critical role by inducing interferon-stimulated genes (ISGs) that help control viral infections. GQH-AII provides a comprehensive tool for examining the expression profiles of key genes involved in the antiviral innate immune response, including a broad range of genes, encompassing PRRs, Type I, II and III interferons, ISGs, and various regulatory genes. Brief examples of how genes may be grouped according to their functions are shown below:
Receptors: TLR3, TLR7, TLR8, IFNLR1, IFNAR1, IL10RB, DHX58, RIGI
Receptor Transducers: FADD, MYD88, TRAF6, IRAK1
Interferons: IFNA1, IFNB1, IFNL1, IFNL2/3, IFNG
Transcription Factors: FOS, JUN, IRF1, IRF3, IRF7, STAT1
ISGs: ISG15, IFIT1, IFIT2, IFITM1, IFITM2, IFITM3
Note:- all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC). GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg ^2+ , and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GQH-AII provides a comprehensive tool for examining the expression profiles of key genes involved in the antiviral innate immune response, including a broad range of genes, encompassing PRRs, Type I, II and III interferons, ISGs, and various regulatory genes.
