EcoPlexTM Mouse Inflammation 6-Plex Panel Kit

3 h

Cross-reactivity of analytes in the Panel: Negligible

Intra-assay CV<10% Inter-assay CV<20%

70%-130%

TNF-α<0.5pg/mL IL-12p70<1pg/mL IFN-γ<1pg/mL MCP-1<1pg/mL IL-6<3pg/mL IL-10<2pg/mL

Mouse

EcoPlexTM CBA kits utilize antibodies-conjugated beads which are different in size and mean fluorescent intensity of labeling dye to capture antigens in sample. After capture beads and sample incubation, biotin-conjugated detection antibodies are added to react with antigen-beads complexes, followed by a streptavidin-R-phycoerythrin (SA-PE) treatment. The fluorescent intensity of PE on each bead populations is quantified by flow cytometer with either a single 488nm laser or dual 488nm and 633/640nm lasers. The maximum emission of the bead classification dye is at 700nm. The concentrations of protein interested are determined by comparing the fluorescent intensities to those of standard curve, like sandwich ELISA data analysis.

Serum,plasma,bodily fluid,cell culture supernatant

TNF-α:P06804 IL-12p70:P43431P43432 IFN-γ:P01580 MCP-1:P10148 IL-6:P08505 IL-10:P18893

This kit is for detection of IFN-γ, IL-6, IL-10, IL-12p70, MCP-1 and TNF-α in mouse serum, plasma, biological fluid and cell culture supernatant.