AdipoGen Life Sciences

TRAIL, Soluble (human) (rec.)

Product Code:
 
AG-40B-0003
Product Group:
 
Recombinant Proteins
Host Type:
 
E.coli
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Shipping:
 
Blue Ice
Storage:
 
-20°C
 

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AG-40B-0003-C01010 ug£80.00
Quantity:
AG-40B-0003-50105 x 10 ug£220.00
Quantity:
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Further Information

Alternate Names/Synonyms:
Apo-2L; TNFSF10; CD253
Biological Activity:
Induces apoptosis of human Jurkat T cells in a concentration range of 5-50ng/ml in the presence of 1µg/ml TNF Ligands Enhancer (Prod. No. AG-35B-0001). In the absence of TNF Ligands Enhancer, TRAIL, Soluble (human) (recombinant) is poorly active.
Concentration:
0.1mg/ml after reconstitution.
EClass:
32160000
Endotoxin:
<0.01EU/µg purified protein (LAL test; Lonza).
Form (Short):
liquid
Formulation:
Lyophilized. Contains PBS.
Handling Advice:
After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1% BSA should be used for further dilutions.
Long Description:
Protein. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a FLAG®-tag. Source: E. coli. Endotoxin content: <0.01EU/µg purified protein (LAL test; Lonza). Lyophilized. Contains PBS. Purity: >95% (SDS-PAGE). TNF-related apoptosis-inducing ligand (TRAIL; Apo2L;CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to fi ve distinct receptors: TRAIL-R1 (DR4; Apo2;CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A;TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1;LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2;TRUNDD; CD264; TNFRSF10D), and osteoprotegerin (OPG; OCIF; TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducingthe formation of the so-called multiprotein death-inducing signaling complex (DISC).
Molecular Weight:
~23kDa (SDS-PAGE)
NCBI, Uniprot Number:
P50591
Package Type:
Plastic Vial
Product Description:
TNF-related apoptosis-inducing ligand (TRAIL; Apo2L;CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to fi ve distinct receptors: TRAIL-R1 (DR4; Apo2;CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A;TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1;LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2;TRUNDD; CD264; TNFRSF10D), and osteoprotegerin (OPG; OCIF; TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducingthe formation of the so-called multiprotein death-inducing signaling complex (DISC).
Purity:
>95% (SDS-PAGE)
Sequence:
The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a FLAG®-tag.
Source / Host:
E. coli
TAGs:
FLAG
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

References

TNF-Related Apoptosis-Inducing Ligand (TRAIL) - Armed Exosomes Deliver Proapoptotic Signals to Tumor Site: L. Rivoltini, et al.; Clin. Cancer Res. 22, 3499 (2016) | Silencing of Mcl-1 overcomes resistance of melanoma cells against TRAIL-armed oncolytic adenovirus by enhancement of apoptosis: B. Tolksdorf, et al.; J. Mol. Med. ahead of print (2021)