FibroFectagen

The delivery of foreign DNA into eukaryotic cells is one of the most common molecular biology techniques to study biological mechanisms. However, unlike transformed cell lines, the efficient transfection of primary cells can be a problem. FibroFectagen is a cationic polymer-based transfection system specifically designed and optimized for efficient transfection of primary fibroblasts. Transfection with FibroFectagen can be carried out in the presence of antibiotics and serum. Instead of normal two-day transfection, an optimized one-day transfection procedure can be performed for time-saving and highly reproducible transfection. One ml of FibroFectagen reagent is sufficient for up to 200 transfections per well in 96-well plate.

Upon receipt, aliquot and store FibroFectagen reagent A at -20°C, avoid repeated freezing/thawing cycles. Once thawed, store FibroFectagen reagent A at 4°C and use in a month. FibroFectagen reagent B can be kept at 4°C.