WST-1 Cell Viability & Proliferation Assay

The reduction of tetrazolim salts to colored formazan compounds by succinate-tetrazolim reductase provides a sensitive and accurate method to measure cell viability and proliferation. The cleavage of the tetrazolim salts to formazan takes place in the mitochondria of metabolically active cells. ScienCell's WST-1 Cell Viability & Proliferation Assay utilizes a tetrazolim salt, WST-1[2-(4Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium]. WST-1 produces a highly water soluble formazan upon metabolically active cells, allowing a direct and user-friendly colorimetric measurement of cell viability and proliferation without an additional extraction step. The WST kit provides the user the flexibility to record absorbance at different time points, if desired.

This assay kit is used to evaluate the cell viability and proliferation in vitro . It is not approved for human or animal use, or application in clinical or in vitrodiagnostic procedures.