Cardiac Myocyte Medium

Cardiac Myocyte Medium (CMM), when used with Cardiac Myocyte Growth Supplement (CMGS, Cat #6252) and 25 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal cardiac myocytes in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal cardiac myocytes in vitro.

CMM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of cardiac myocyte growth supplement (CMGS, Cat. No. 6252) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).