Keratinocyte Medium

Keratinocyte Medium (KM), when used with Keratinocyte Growth Supplement (KGS, Cat #2152), is a complete medium designed for optimal growth of normal keratinocytes in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal keratinocytes in vitro.

KM consists of 500 ml of basal medium, 5 ml of keratinocyte growth supplement (KGS, Cat. No. 2152) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).