Mesenchymal Stem Cell Osteogenic Differentiation Medium

Mesenchymal Stem Cell Osteogenic Differentiation Medium (MODM), when used with Mesenchymal Stem Cell Osteogenic Differentiation Supplement (MODS, Cat #7532) and 25 ml of fetal bovine serum, is a complete medium designed for the optimal osteogenic differentiation of mesenchymal stem cell (MSCs) in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals, and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively promotes osteogenic differentiation of normal human mesenchymal stem cells in vitro .

MODM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. #0025), 5 ml of Mesenchymal Stem Cell Osteogenic Differentiation Supplement (MODS, Cat. #7532), and 5 ml of penicillin/streptomycin solution (P/S, Cat. #0503). Note: FBS, MODS, and P/S are not pre-mixed in MODM; they must be added separately to make the complete MODM. There will be an additional 8% custom fee applied to the phenol red free version of this product. - The phenol red free version of this product requires a minimum purchase of 20 Bottles and a 3 week estimated time of arrival. -