Sciencell

NAD/NADH Assay

Product Code:
 
SC-8368
Product Group:
 
Cell Based Assays
Supplier:
 
Sciencell
Host Type:
 
Human
Regulatory Status:
 
RUO
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SC-8368100 tests£412.00
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Further Information

Description:
Nicotinamide adenine dinucleotide (NAD), which acts as soluble electron carriers between proteins, is an important enzymatic cofactor involved in many redox reactions. NAD functions in modulating cellular redox status by controlling signaling and transcriptional events, making it and related enzymes drug targets for various metabolic disorders. This colorimetric assay is based on lactate dehydrogenase catalyzed reduction of NAD, in which the formed NADH could reduce tetrazolium salt INT to an INT-formazan product. The intensity of the INT-formazan product, which exhibits an absorbance maximum at 490 nm, is proportional to the amount of NADt (NAD and NADH) or NADH in the sample. This assay is specific for NAD and NADH, and does not detect NADP or NADPH.
Extra Description:
NAD/NADH Assay kit could measure NADt (NAD and NADH) and NADH concentrations and NAD/NADH ratio in cell and tissue extracts. This product is for research purposes only and not for use in animals, humans, or diagnostic procedures.

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