NAPRTase (human) (rec.) (His)

Nicotinate Phosphoribosyltransferase; FHA-HIT-interacting Protein; FHIP; EC 2.4.2.11

0.043µmol/min/mg: hNAPRTase activity was measured by a custom coupled enzymatic assay which uses a secondary enzyme for the quantification of the PRPP consumed by hNAPRTase. The reaction buffer contained 50 mM Hepes pH 7.5, 500 µM PRPP, 100 µM nicotinic acid, 20 mM MgCl2, 2.5 mM DTT and hNAPRTase at 5 µM, 25°C, spectrophotometrical monitoring.

0.5mg/ml (Lot specific)

32160000

N/A

liquid

Liquid. In 50mM HEPES/KOH pH 7.5 containing 300mM potassium chloride and 10mM DTT.

After opening, prepare aliquots and store at -80°C.Avoid freeze/thaw cycles.

Protein. Human NAPRTase is fused at the N-terminus to a His-tag. Source: E. coli. Endotoxin content: N/A. Liquid. In 50mM HEPES/KOH pH 7.5 containing 300mM potassium chloride and 10mM DTT. Purity: >98% (SDS-PAGE). Human nicotinate phosphoribosyltransferase (NAPRTase) is localized in the cytoplasm and is involved in biological processes such as NAD biosynthetic and metabolic processes, nicotinamide metabolic process, nicotinate nucleotide salvage, response to oxidative stress and water-soluble vitamin metabolic process. It functions by catalyzing the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN) and is essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells. It is a crucial factor in the NAD+ biosynthesis pathway. Catalytic activity: Beta-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate.

~58.0kDa

Q6XQN6

Plastic Vial

Human nicotinate phosphoribosyltransferase (NAPRTase) is localized in the cytoplasm and is involved in biological processes such as NAD biosynthetic and metabolic processes, nicotinamide metabolic process, nicotinate nucleotide salvage, response to oxidative stress and water-soluble vitamin metabolic process. It functions by catalyzing the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN) and is essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells. It is a crucial factor in the NAD+ biosynthesis pathway. Catalytic activity: Beta-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate.

>98% (SDS-PAGE)

Human NAPRTase is fused at the N-terminus to a His-tag.

E. coli

His

Non-hazardous

Other Enzymes

12352204

Stable for at least 6 months after receipt when stored at -80°C.