ProSci

Apaf1 Antibody

Product Code:
 
PSI-2013
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 1 and (B) 2 μg/ml.
2 / 3
Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 μg/mL.
3 / 3
Immunofluorescence of Apaf1 in K562 cells with Apaf1 antibody at 10 μg/mL.
❮❯

Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 μg/mL.
Immunofluorescence of Apaf1 in K562 cells with Apaf1 antibody at 10 μg/mL.

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CodeSizePrice
PSI-2013-0.02mg0.02mg£150.00
Quantity:
PSI-2013-0.1mg0.1mg£449.00
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Further Information

Additional Names:
Apaf1 Antibody: CED4, APAF-1, KIAA0413, Apoptotic protease-activating factor 1
Application Note:
Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 μg/mL. A 115 - 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Apaf1 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.
Background References:
  • Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997;90:405-13
  • Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998;94:727-37
  • Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation ofApaf1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89
  • Hu Y, Benedict MA, Wu D, Inohara N, Nunez G. Bcl-XL interacts with Apaf1 and inhibits Apaf1-dependent caspase-9 activation. Proc Natl Acad Sci USA 1998;95:4386-91 (RD1299)
Buffer:
Apaf1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1.

The sequences of the immunogenic peptide are identical between human and mouse.

The immunogen is located within the first 50 amino acids of Apaf1.
NCBI Gene ID #:
317
NCBI Official Name:
apoptotic peptidase activating factor 1
NCBI Official Symbol:
APAF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 132 kDa

Observed: 130 kDa
Protein Accession #:
AAC51678
Protein GI Number:
2330015
Purification:
Apaf1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Cancer
Swissprot #:
O14727
User NOte:
Optimal dilutions for each application to be determined by the researcher.