ADAM10 Antibody
PSI-2051
Rabbit
IgG
Polyclonal
RUO
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
No additional charges, what you see is what you pay! *
Code | Size | Price |
---|
PSI-2051-0.02mg | 0.02mg | £150.00 |
PSI-2051-0.1mg | 0.1mg | £449.00 |
* Rare exceptions are clearly labelled (only 0.14% of items!).
Typical lead time: 14-21 working days.
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Further Information
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- Rosendahl et al. J Biol Chem 1997;272:24588-93.
- Pan and Rubin. Cell 1997;90:271-80.
- Qi et al. Science 1999;283:91-4.
- Howard et al. Biochem J 1996;317:45-50. (RD1299).
The immunogen is located within the last 50 amino acids of ADAM10.
KO validation (Figure 1 and 2): Anti-ADAM10 antibody (2051) specificity was further verified by ADAM10 specific knockout. ADAM10 signal in was detected by 2051 in wild type of MEF cells and 293 cells, but not in ADAM10 knockout cells.
Independent Antibody Validation in Cell lines (Figure 3) shows similar ADAM10 expression profile in human and mouse cell lines detected by two independent anti-ADAM10 antibodies that recognize different epitopes, 2051 against C-terminus domain and 24-024 against human recombinant fusion protein. ADAM10 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.
Regulated expression validation (Figure 12 and 14): ADAM10 expression detected by anit-ADAM10 antibodies (2051) was down-regulated when exposed to oxygen-glucose deprivation (OGD) or glutamate (Fig 8), and up-regulated in membrane compartment whereas down-regulated in intracellular fractions when treated with donepezil (Fig 14).
KD validation (Figure 15): Anti-ADAM10 antibody (2051) specificity was further verified by ADAM10 specific knockdown. ADAM10 signal in HEK/APP cells transfected with ADAM10 siRNA or Ferrochelatase siRNA (F) or N-methylprotoporphyrin IX siRNA (N) was disrupted in comparison with that in mock-transfected cells (M) or cells transfected with TACE siRNA (T).
Documents
References
- Al-Tamimi et al. Pathologic shear triggers shedding of vascular receptors: a novel mechanism for down-regulation of platelet glycoprotein VI in stenosed coronary vessels. Blood. 2012;119(18):4311-20. PMID: 22431567
- Hurtado et al. TACE/ADAM17-TNF-alpha pathway in rat cortical cultures after exposure to oxygen-glucose deprivation or glutamate. J Cereb Blood Flow Metab. 2002;22(5):576-85.PMID: 11973430
- Coma et al. Oxidative stress triggers the amyloidogenic pathway in human vascular smooth muscle cells. Neurobiol Aging. 2008;29(7):969-80. PMID: 17306421
- Mosser et al. The adipocyte differentiation protein APMAP is an endogenous suppressor of A? production in the brain. Hum Mol Genet. 2015;24(2):371-82. PMID: 25180020
- Zimmermann et al. Acetylcholinesterase inhibitors increase ADAM10 activity by promoting its trafficking in neuroblastoma cell lines. J Neurochem. 2004;90(6):1489-99.PMID: 15341532
- Gatta et al. Inhibition of heme synthesis alters Amyloid Precursor Protein processing. J Neural Transm (Vienna). 2009;116(1):79-88. PMID: 19002554
- Zimmermann et al. Cholinesterase inhibitors influence APP metabolism in Alzheimer disease patients. Neurobiol Dis. 2005;19(1-2):237-42. PMID: 15837579
- Kabacik and Raj. Ionising radiation increases permeability of endothelium through ADAM10-mediated cleavage of VE-cadherin. Oncotarget. 2017;8(47):82049-82063. PMID: 29137243
- Colciaghi et al. [alpha]-Secretase ADAM10 as well as [alpha]APPs is reduced in platelets and CSF of Alzheimer disease patients. Mol Med. 2002;8(2):67-74. PMID: 12080182
- Bandyopadhyay et al. Interleukin-1alpha stimulates non-amyloidogenic pathway by alpha-secretase(ADAM-10 and ADAM-17) cleavage of APP in human astrocytic cells involving p38 MAP kinase. J Neurosci Res. 2006;84(1):106-18. PMID: 16724341
- Hiltunen et al. Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion. J Biol Chem. 2006;281(43):32240-53. PMID: 16945923
- Harjes et al. Fatty acid-binding protein 4, a point of convergence for angiogenic and metabolic signaling pathways in endothelial cells. J Biol Chem. 2014;289(33):23168-76. PMID: 24939870
- Levin-Allerhand et al. 17Alpha-estradiol and 17beta-estradiol treatments are effective in lowering cerebral amyloid-beta levels in AbetaPPSWE transgenic mice. J Alzheimers Dis. 2002;4(6):449-57. PMID: 12515896
- Peng et al. Huperzine A regulates amyloid precursor protein processing via protein kinase C and mitogen-activated protein kinase pathways in neuroblastoma SK-N-SH cells over-expressing wild type human amyloid precursor protein 695. Neuroscience. 2007;150(2):386-95. PMID: 17945434
- Smith et al. Compositions and methods for inhibiting beta amyloid secretion. U.S. Patent application publication. 2013. Pub. No.: US 2013/0158112 A1.
- Can Zhang. Identifying Genes That Encode APP Metabolism Modulators On Chromosome 9q22. Drexel University, Philadelphia, PA. PhD Thesis, 2007.
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