Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
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Further Information
TMEM135 Antibody: PMP52, Transmembrane protein 135, Peroxisomal membrane protein 52, PMP52
TMEM135 Antibody can be used for detection of TMEM135 by Western blot starting at 1 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
TMEM135 Antibody: The transmembrane protein 135 (TMEM135), also known as peroxisomal membrane protein 52 (PMP52), was initially identified as a protein that might be critical for adipogenesis and osteoblastogenesis. Further studies have indicated that TMEM135 may be part of a regulatory circuit that plays an important role in fat metabolism and energy expenditure in both C. elegans as well as mammalian organisms. Finally, recent experiments has suggested that TMEM135 may be an additional driver of breast cancer.
- Scheideler M, Elabd C, Zaragosi LE, et al. Comparative transcriptomics of human multipotent stem cells during adipogenesis and osteoblastogenesis. BMC Genomics 2008; 9:340.
- Exil VJ, Avila DS, Benedetto A, et al. Stress-induced TMEM135 protein is part of a conserved genetic network involved in fat storage and longetivity regulation in Caenorhabditis elegans. PLoS One 2010; 5:e14228.
- Natrajan R, Mackay A, Lambros MB, et al. A whole-genome massively parallel sequencing analysis of BRCA1 mutant oestrogen receptor-negative and ?positive breast cancers. J. Pathol. 2012; 227:29-41.
TMEM135 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
TMEM135 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human TMEM135 .
The immunogen is located within the last 50 amino acids of TMEM135.
65084
transmembrane protein 135
TMEM135
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 50 kDa
Observed: 53 kDa
NP_075069
281182571
TMEM135 Antibody is affinity chromatography purified via peptide column.
Obesity
At least two isoforms of TMEM135 are known to exist; this antibody will detect both isoforms.
Q86UB9
Optimal dilutions for each application to be determined by the researcher.