Further Information
B-raf Antibody: NS7, BRAF1, RAFB1, B-RAF1, Proto-oncogene B-Raf
WB: 0.5-2 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
B-raf Antibody: B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.
- McCubrey et al. Biophys. Acta 2007; 1773:1263-84.
- Madhunapantula and Robertsonet al. Cancer Res.2008; 68:5-8.
- Sarkozy et al. Hum. Mutat.2009; 30:695-702.
B-raf Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Rat: (100%), Chicken: (100%)
Anti-B-raf antibody (7379) was raised against a peptide corresponding to 19 amino acids near the center of human B-raf.
The immunogen is located within amino acids 330 - 380 of B-raf.
Human B-raf has one isoform (766aa, 84kD). Mouse B-raf has 2 isoforms, including isoform 1 (804aa, 89kD) and isoform 2 (329aa, 37kD). Rat B-raf a has one isoform (712aa, 80kD). 7379 can detect in human B-raf and mouse isoform 1.
673
v-raf murine sarcoma viral oncogene homolog B1
BRAF
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 94kD
Observed: 94 kD
P15056
50403720
B-raf Antibody is affinity chromatography purified via peptide column.
Cancer,Cell Cycle
This antibody will not cross-react with C-raf.
P15056
Optimal dilutions for each application to be determined by the researcher.
Independent Antibody Validation in Cell lines (Figure 2) shows similar B-raf expression profile in human and mouse cell lines detected by two independent anti-B-raf antibodies that recognize different epitopes, 5777 against central domain and 7379 against a slight different central domain. B-raf proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.