Further Information
TRIM24 Antibody: PTC6, TF1A, TIF1, RNF82, TIF1A, hTIF1, TIF1ALPHA, Transcription intermediary factor 1-alpha, E3 ubiquitin-protein ligase TRIM24, TIF1-alpha
TRIM24 antibody can be used for detection of TRIM24 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
TRIM24 Antibody: TRIM24 (TIF1), a member of the tripartite motif (TRIM) family, plays a role in the regulation of cell proliferation and apoptosis. TRIM24 localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The TRIM motif includes three zinc-binding domains (RING, B-box type 1 and B-box type 2) and a coiled-coil region. TIF1 mediates transcriptional events by interactions with the AF2 region of several nuclear receptors, such as the estrogen, retinoic acid and vitamin D3 receptors. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC).
- Fraser RA, Heard DJ, Adam S, et al. The putative cofactor TIF1 is a protein kinase that is hyperphosphorylated upon interaction with liganded nuclear receptors. J. Biol. Chem. 1998; 273:16199-204.
- Tisserand J, Khetchoumian K, Thibault C, et al. Tripartite motif 24 (Trim24/Tif1a) tumor suppressor protein is a novel negative regulator of interferon (IFN)/signal transducers and activators of transcription (STAT) signaling pathway acting through retinoic acid receptor a (Rara) inhibition. J. Biol. Chem. 2011; 286:33369-79.
- Herquel B, Ouararhni K, Khetchoumian K, et al. Transcription cofactors TRIM24, TRIM28, and TRIM33 associate to form regulatory complexes that suppress murine hepatocellular carcinoma. Proc. Natl. Acad. Sci. USA 2011; 108:8212-7.
- Klugbauer S and Rabes HM. The transcription co-activator HTIF1 and a related protein are fused to the RET receptor tyrosine kinase in childhood papillary thyroid carcinomas. Oncogene 1999;18:4388-93.
TRIM24 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
TRIM24 antibody was raised against a 19 amino acid peptide near the center of human TRIM24.
The immunogen is located within amino acids 530 - 580 of TRIM24.
8805
tripartite motif containing 24
TRIM24
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 112, 116
Observed: 108, 1 of 2 isoforms
NP_056989
47419911
TRIM24 Antibody is affinity chromatography purified via peptide column.
Apoptosis
At least two isoforms of TRIM24 are known to exist.
O15164
Optimal dilutions for each application to be determined by the researcher.