ProSci

TRIM33 Antibody

Product Code:
 
PSI-7385
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of TRIM33 in human liver tissue lysate with TRIM33 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunohistochemistry of TRIM33 in human liver tissue with TRIM33 antibody at 2.5 μg/ml.
3 / 3
Immunofluorescence of TRIM33 in human liver tissue with TRIM33 antibody at 20 μg/ml.
❮❯

Western blot analysis of TRIM33 in human liver tissue lysate with TRIM33 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of TRIM33 in human liver tissue with TRIM33 antibody at 2.5 μg/ml.
Immunofluorescence of TRIM33 in human liver tissue with TRIM33 antibody at 20 μg/ml.

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CodeSizePrice
PSI-7385-0.02mg0.02mg£150.00
Quantity:
PSI-7385-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
TRIM33 Antibody: ECTO, PTC7, RFG7, TF1G, TIF1G, TIFGAMMA, TIF1GAMMA, KIAA1113, E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, Protein Rfg7
Application Note:
TRIM33 antibody can be used for detection of TRIM33 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TRIM33 Antibody: TRIM24 (TIF1alpha), TRIM28 (TIF1beta), and TRIM33 (TIF1gamma) are three related cofactors belonging to the tripartite motif superfamily that interact with distinct transcription factors (1-2). The structure of TRIM33 is similar to TRIM24 and TRIM28, exhibiting multiple domains (RING finger, coiled coil, B boxes, PHD/TTC, and bromodomain) (3). TRIM33 functions in cell differentiation and development, playing a role in differentiation of hematopoietic cells (3). It interacts with the Smad2/3 transcription factor in hematopoietic, mesenchymal, and epithelial cell types to mediate different transcriptional effects in response to TGF-beta (4). It acts as an E3 ubiquitin-protein ligase and promotes SMAD4 ubiquitination (5).
Background References:
  • Ransom DG, Bahary N, Niss K, et al. The zebrafish moonshine gene encodes transcriptional intermediary factor 1gamma, an essential regulator of hematopoiesis. PLoS Biol. 2004; 2:E237.
  • Herquel B, Ouararhni K, Khetchoumian K, et al. Transcription cofactors TRIM24, TRIM28, and TRIM33 associate to form regulatory complexes that suppress murine hepatocellular carcinoma. Proc. Natl. Acad. Sci. USA 2011; 108:8212-7.
  • He W, Dorn DC, Erdjument-Bromage H, et al. Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of the TGFbeta pathway. Cell 2006; 125:929-41.
  • Dupont S, Inui M, Newfeld SJ. Regulation of TGF-β signal transduction by mono- and deubiquitylation of Smads. FEBS Lett. 2012; 586:1913-20.
Buffer:
TRIM33 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TRIM33 antibody was raised against an 18 amino acid peptide near the center of human TRIM33.

The immunogen is located within amino acids 580 - 630 of TRIM33.
NCBI Gene ID #:
51592
NCBI Official Name:
tripartite motif containing 33
NCBI Official Symbol:
TRIM33
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 70, 124 kDa

Observed: 70 kDa
Protein Accession #:
NP_056990
Protein GI Number:
74027249
Purification:
TRIM33 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
TRIM33 antibody is human, mouse and rat reactive. At least two isoforms of TRIM33 are known to exist.
Swissprot #:
Q9UPN9
User NOte:
Optimal dilutions for each application to be determined by the researcher.