ProSci

DAP Antibody

Product Code:
 
PSI-7389
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of DAP in human small intestine tissue lysate with DAP antibody at 1 μg/mL.
2 / 3
Immunohistochemistry of DAP in human small intestine tissue with DAP antibody at 5 μg/ml.
3 / 3
Immunofluorescence of DAP in human small intestine tissue with DAP antibody at 20 μg/ml.
❮❯

Western blot analysis of DAP in human small intestine tissue lysate with DAP antibody at 1 μg/mL.
Immunohistochemistry of DAP in human small intestine tissue with DAP antibody at 5 μg/ml.
Immunofluorescence of DAP in human small intestine tissue with DAP antibody at 20 μg/ml.

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CodeSizePrice
PSI-7389-0.02mg0.02mg£150.00
Quantity:
PSI-7389-0.1mg0.1mg£449.00
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Further Information

Additional Names:
DAP Antibody: DAP1, Death-associated protein 1, DAP-1
Application Note:
DAP antibody can be used for detection of DAP by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).
Background References:
  • Deiss LP, Feinstein E, Berissi H, et al. Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death. Genes Dev. 1995; 9:15-30.
  • Koren I, Reem E, and Kimchi A. DAP1, a novel substrate of mTOR, negatively regulates autophagy. Curr. Biol. 2010; 20:1093-8.
Buffer:
DAP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (89%)
Immunogen:
DAP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DAP.

The immunogen is located within the last 50 amino acids of DAP.
NCBI Gene ID #:
1611
NCBI Official Name:
death-associated protein
NCBI Official Symbol:
DAP
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 11 kDa

Observed: 11 kDa
Protein Accession #:
NP_004385
Protein GI Number:
4758120
Purification:
DAP Antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
SPECIFICITY:
DAP antibody is human, mouse and rat reactive.
Swissprot #:
P51397
User NOte:
Optimal dilutions for each application to be determined by the researcher.