Further Information
Vinculin Antibody : MV, MVCL, CMD1W, CMH15, HEL114, Vinculin, Metavinculin, MV
WB: 1-2 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in rat samples; Immunofluorescence in rat samples. All other applications and species not yet tested.
Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).
- Burridge et al. Annu. Rev. Cell Biol.1988; 4:487-525.
- Gilmore et al. J. Cell Sci. 1992; 103:719-31.
- Deakin et al. PLoS One 2012; 7:e37990.
- Goldmann et al. Cell Biol. Int. 2013; Feb 1.
Vinculin Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (100%)
Anti-Vinculin antibody (7489) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human Vinculin.
The immunogen is located within amino acids 1060-1110 of Vinculin.
Human Vinculin has 3 isoforms, including isoform 2 (1134aa, 124kD), isoform 1 (1066aa, 117kD) and isoform 3 (222aa, 25kD). Mouse Vinculin has one isoform (1066aa, 117kD) and Rat Vinculin also has one isoform (1066aa, 117kD). 7489 can detect human, mouse and rat.
7414
vinculin
VCL
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 124kD
Observed: 124kD
NP_003364
4507877
Vinculin Antibody is affinity chromatography purified via peptide column.
Homeostasis
Vinculin antibody is human, mouse and rat specific. At least three isoforms of Vinculin are known to exist.
P18206
Optimal dilutions for each application to be determined by the researcher.
Independent Antibody Validation in Cell lines (Figure 2) shows similar Vinculin expression profile in human, mouse and rat cell lines detected by three independent anti-Vinculin antibodies that recognize different epitopes, 7489 against C-terminus domain, 7807 against N-terminus domain and 7809 against central domain. Vinculin proteins are detected in the most tested cell lines at different expression levels by the three independent antibodies.