ProSci

KDM1B Antibody

Product Code:
 
PSI-7535
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
1 / 1
Western blot analysis of KDM1B in K562 cell lysate with KDM1B antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide
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Western blot analysis of KDM1B in K562 cell lysate with KDM1B antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide

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CodeSizePrice
PSI-7535-0.02mg0.02mg£150.00
Quantity:
PSI-7535-0.1mg0.1mg£449.00
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Further Information

Additional Names:
KDM1B Antibody: AOF1, LSD2, C6orf193, bA204B7.3, dJ298J15.2, AOF1, Lysine-specific histone demethylase 1B, Flavin-containing amine oxidase domain-containing protein 1
Application Note:
KDM1B antibody can be used for detection of KDM1B by Western blot at 0.5 μg/ml.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
Flavin-dependent histone demethylases regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (reviewed in 1). KDM1B is a recently identified histone H3K4 demethylase that is required to establish maternal genomic imprints; targeted disruption of the KDM1B gene in mice led to lethality in their embryos, suggesting demethylation of H3K4 is critical for establishing the DNA methylation imprints (2). KDM1B has also been shown to play a role in the transcription elongation in several genes (3).
Background References:
  • Culhane JC and Cole PA. LSD1 and the chemistry of histone demethylation. Curr. Opin. Chem. Biol. 2007; 11:561-8.
  • Ciccone DN, Su H, Hevi S, et al. KDM1B is a histone H3K4 demethylase required to establish maternal genomic imprints. Nature 2009; 461:415-8.
  • Fang R, Barbera A, Xu Y, et al. Human LSD2/KDM1b/AOF1 regulates genes transcription by modulating intragenic H3K4me2 methylation. Mol. Cell 2010; 39:222-233.
Buffer:
KDM1B antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
KDM1B antibody was raised against a 17 amino acid peptide near the amino terminus of human KDM1B.

The immunogen is located within amino acids 80 - 130 of KDM1B.
NCBI Gene ID #:
221656
NCBI Official Name:
lysine (K)-specific demethylase 1B
NCBI Official Symbol:
KDM1B
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 90 kDa

Observed: 90 kDa
Protein Accession #:
NP_694587
Protein GI Number:
116256451
Purification:
KDM1B antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
KDM1B antibody is human, mouse and rat reactive. At least two isoforms of KDM1B are known to exist; this KDM1B antibody will detect both isoforms.
Swissprot #:
Q8NB78
User NOte:
Optimal dilutions for each application to be determined by the researcher.