ProSci

MAP1LC3A Antibody

Product Code:
 
PSI-7579
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
1 / 3
Western blot analysis of MAP1LC3A in 293 cell lysate with MAP1LC3A antibody at 1 μg/ml.
2 / 3
Immunohistochemistry of MAP1LC3A in human brain tissue with MAP1LC3A antibody at 5 μg/mL.
3 / 3
Immunofluorescence of MAP1LC3A in human brain tissue with MAP1LC3A antibody at 20 μg/mL.
❮❯

Western blot analysis of MAP1LC3A in 293 cell lysate with MAP1LC3A antibody at 1 μg/ml.
Immunohistochemistry of MAP1LC3A in human brain tissue with MAP1LC3A antibody at 5 μg/mL.
Immunofluorescence of MAP1LC3A in human brain tissue with MAP1LC3A antibody at 20 μg/mL.

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PSI-7579-0.02mg0.02mg£150.00
Quantity:
PSI-7579-0.1mg0.1mg£449.00
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Further Information

Additional Names:
MAP1LC3A Antibody: LC3, LC3A, ATG8E, MAP1ALC3, MAP1BLC3Autophagy-related protein LC3 A
Application Note:
MAP1LC3A antibody can be used for detection of MAP1LC3A by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3A belongs to the MAP1 LC3 family of proteins that form mature complexes with MAP1A and MAP1B which are thought to be important in the formation and development of axons and dendrites (2). MAP1LC3A is one of three isoforms of MAP1LC3, the mammalian homolog of yeast ATG8, an essential autophagy protein. These isoforms exhibit distinct expression patterns and MAP1LC3A, like MAP1LC3A but not MAP1LC3B, is post-translationally modified, suggesting the three isoforms may have different physiological functions (3).
Background References:
  • Mandelkow E and Mandelkow EM. Microtubules and microtubule-associated proteins. Curr. Opin. Cell Biol. 1995; 7:72-81.
  • Halpain S and Dehmelt L. The MAP1 family of microtubule-associated proteins. Genome Biol. 2006; 7:224.
  • He H, Dang Y, Dai F, et al. Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B. J. Biol. Chem. 2003; 278:29278-87.
Buffer:
MAP1LC3A antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MAP1LC3A antibody was raised against a 10 amino acid peptide near the amino terminus of human MAP1LC3A.

The immunogen is located within the first 50 amino acids of MAP1LC3A.
NCBI Gene ID #:
84557
NCBI Official Name:
microtubule-associated protein 1 light chain 3 alpha
NCBI Official Symbol:
MAP1LC3A
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 14 kDa

Observed: 17 kDa
Protein Accession #:
NP_852610
Protein GI Number:
31563518
Purification:
MAP1LC3A antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
SPECIFICITY:
MAP1LC3A antibody is human specific. At least two isoforms of MAP1LC3A are known to exist. MAP1LC3A antibody is predicted to not cross-react with MAP1LC3B or MAP1LC3C.
Swissprot #:
Q9H492
User NOte:
Optimal dilutions for each application to be determined by the researcher.