Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
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Further Information
PHC3 Antibody: EDR3, HPH3, EDR3, PH3, Polyhomeotic-like protein 3, Early development regulatory protein 3, hPH3
PHC3 antibody can be used for detection of PHC3 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Polycomb group (PcG) proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes throughout development (1). PHC3 (Polyhomeotic-like protein 3), also known as Early development regulatory protein 3, is a 983 amino acid nuclear protein that is a component of the PcG multiprotein PRC1 complex (2). PRC1 complex acts via chromatin remodeling and modification of histones. PHC3 contains 1 FCS-type zinc finger and SAM (sterile alpha motif) domain (2-3). PHC 3 has been regarded as a candidate tumor suppressor of osteosarcoma (4).
- Valk-Lingbeek ME, Bruggeman SW, and Van Lohuizen M. Stem cells and cancer; the polycomb connection. Cell 2004; 118:409?18.
- Deshpande AM, Akunowicz JD, Reveles XT, et al. PHC3, a component of the hPRC-H complex, associates with E2F6 during G0 and is lost in osteosarcoma tumors. Oncogene 2007; 26:1714-22.
- Isono K, Fujimura Y, Shinga J, et al. Mammalian polyhomeotic homologues Phc2 and Phc1 act in synergy to mediate polycomb repression of Hox genes. Mol. Cell Biol. 2005; 25:6694-706.
- Iwata S, Takenobu H, Kageyama H, et al. Polycomb group molecule PHC3 regulates polycomb complex composition and prognosis of osteosarcoma. Cancer Sci. 2010; 101:1646-52.
PHC3 antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
PHC3 antibody was raised against a 19 amino acid peptide near the amino terminus of human PHC3.
The immunogen is located within amino acids 240 - 290 of PHC3.
80012
polyhomeotic homolog 3 (Drosophila)
PHC3
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 109 kDa
Observed: 120 kDa
NP_079223
148612879
PHC3 antibody is affinity chromatography purified via peptide column.
Stem Cell
PHC3 antibody is human, mouse and rat specific. At least six isoforms of PHC3 are known to exist.
Q8NDX5
Optimal dilutions for each application to be determined by the researcher.