Further Information
SPINSTER Antibody: LAT, nrs, SPIN1, SPINL, HSpin1, PP2030, PP20300, Protein spinster homolog 1
SPINSTER antibody can be used for detection of SPINSTER by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPINSTER, also known as SPNS1 or SPIN1, is a 528 amino acid multi-pass membrane protein that localizes to the inner mitochondrial membrane and belongs to the spinster subfamily of the major facilitator superfamily (1). SPINSTER interacts with Bcl-x and Bcl-2 and, via this interaction, is thought to be involved in necrotic or autophagic cell death (2). The related protein SPNS2 is critical for the normal lymphocyte localization and mammalian immune system function (1,3).
- Saier MH Jr, Beatty JT, Goffeau A, et al. The major facilitator superfamily. J. Mol. Microbiol. Biotechnol. 1999; 1:257-79.
- Yanagisawa H, Miyashita T, Nakano Y, et al. HSpin1, a transmembrane protein interacting with Bcl-2/Bcl-xL, induces a caspase-independent autophagic cell death. Cell Death Differ. 2003; 10:798-807.
- Nakano Y, Fujitani K, Kurihara J, et al. Mutations in the novel membrane protein spinster interfere with programmed cell death and cause neural degeneration in Drosophila melanogaster. Mol. Cell. Biol. 2001; 21:3775-88.
SPINSTER antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Bovine: (83%), Rat: (81%), Mouse: (81%)
SPINSTER antibody was raised against an 18 amino acid peptide near the amino terminus of human SPINSTER.
The immunogen is located within the first 50 amino acids of SPINSTER.
83985
spinster homolog 1 (Drosophila)
SPNS1
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 58 kDa
Observed: 56 kDa
NP_001135920
215490098
SPINSTER antibody is affinity chromatography purified via peptide column.
Autophagy
SPINSTER antibody is human specific. At least four isoforms of SPINSTER are known to exist. This antibody is predicted to not cross-react with other members of the spinster family of proteins.
Q9H2V7
Optimal dilutions for each application to be determined by the researcher.