Further Information
HMGB1 Antibody: HMG1, HMG3, SBP-1, HMG1, High mobility group protein B1, High mobility group protein 1, HMG-1
WB: 1 μg/mL; IHC: 1-2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse, and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested.
The high-mobility group box 1 (HMGB1) protein was originally identified as a highly conserved nuclear DNA-binding protein that participates in DNA replication, repair and transcriptional regulation of gene expression (1,2). However, HMGB1 has more recently emerged as an extracellularly released mediator of inflammation and repair responses in lipopolysaccharide (LPS)-induced endotoxemia and sepsis (3). HMGB1 has also been suggested to induce secretion of pro-inflammatory cytokines and chemokines by inducing NF-kB signaling downstream of Toll-like receptor (TLR) 2 and TLR4 activation (4).
- Smerdon MJ and Isenberg I. Interactions between the subfractions of calf thymus H1 and nonhistone chromosomal proteins HMG1 and HMG2. Biochemistry 1976; 15:4242-7.
- Stros. Biocim. Biophys. Acta. 2010; 1799:101-113.
- Wang et al. Science 1999; 285:248-51.
- Van Zoelenet al. Shock 2009; 31:280-4.
- Wei etal. Cell 2021; 184:76-91.
HMGB1 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Chicken: (100%), Pig: (100%)
Anti-HMGB1 antibody (7715) was raised against a peptide corresponding to 19 amino acids near the center of human HMGB1.
The immunogen is located within 70-120 amino acids of HMGB1.
Human HMGB1 has only 1 isoform (215aa, 25kD). Mouse HMGB1 has only 1 isoform (215aa, 25kD). Rat HMGB1 has only 1 isoform (215aa, 25kD). This antibody detects HMGB1 of human, mouse and rat.
3146
high mobility group box 1
HMGB1
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 25 kDa
Observed: 25 kDa
NP_002119
4504425
HMGB1 antibody is affinity chromatography purified via peptide column.
Innate Immunity
HMGB1 antibody is human, mouse and rat reactive.
P09429
Optimal dilutions for each application to be determined by the researcher.
KO Validation (Figure 1): Anti-HMGB1 antibodies (7715) specificity was further verified by HMGB1 specific knockout cell lysate. HMGB1 signal was detected in wild type cells, but not in the HMGB1 knockout cells.
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