ProSci

Src Antibody

Product Code:
 
PSI-79-176
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store antibody at -20˚C for up to one year.
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Western blot analysis of lysed extracts from HT29 cells untreated or treated with PMA using Src (Phospho-Tyr529).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src (Phospho-Tyr529).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using Src (Phospho-Tyr529).
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Western blot analysis of lysed extracts from HT29 cells untreated or treated with PMA using Src (Phospho-Tyr529).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src (Phospho-Tyr529).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using Src (Phospho-Tyr529).

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PSI-79-176-0.1mg0.1mg£488.00
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Further Information

Additional Names:
ASV, SRC1, c-SRC, p60-Src, PKB, RAC, C-SRC
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.
Background References:
  • Pyper J. M., (1985) Mol. Cell. Biol. 5:831-838Pyper J. M. (1990) Mol. Cell. Biol. 10:2035-2040Xu W., (1997) . Nature 385:595-602Benes C. H., (2005) Cell 121:271-280
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Src (Phospho-Tyr529) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y (p) -Q-P) derived from Human Src.
Modifications:
Phospho-Specific
NCBI Gene ID #:
6714
NCBI Official Name:
v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
NCBI Official Symbol:
SRC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
NP_005408.1
Protein GI Number:
4885609
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of Src only when phosphorylated at tyrosine 529.
Swissprot #:
P12931