ProSci

14-3-3 zeta Antibody

Product Code:
 
PSI-79-188
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store antibody at -20˚C for up to one year.
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Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using 14-3-3 zeta (Phospho-Ser58).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta (Phospho-Ser58).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using 14-3-3 zeta (Phospho-Ser58).
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Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using 14-3-3 zeta (Phospho-Ser58).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta (Phospho-Ser58).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using 14-3-3 zeta (Phospho-Ser58).

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PSI-79-188-0.1mg0.1mg£488.00
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Further Information

Additional Names:
HEL4, YWHAD, KCIP-1, HEL-S-3, 14-3-3-zeta, JNK2, PRKM9, SAPK1A, 1433Z, 143Z, FAS, Factor activating exoenzyme S
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.
Background References:
  • Gu YM, et al. (2006) FEBS Lett ; 580 (1): 305-310Powell DW, et al. (2003) Mol Cell Biol; 23 (15): 5376-5387Mackintosh C. (2004) Biochem J; 381 (Pt 2): 329-342.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
14-3-3 zeta (Phospho-Ser58) antibody was raised against a peptide sequence around phosphorylation site of serine 58 (R-S-S (p) -W-R) derived from Human 14-3-3 zeta.
Modifications:
Phospho-Specific
NCBI Gene ID #:
7534
NCBI Official Name:
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta
NCBI Official Symbol:
YWHAZ
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
28 kDa
Protein Accession #:
NP_001129171.1
Protein GI Number:
208973238
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer,Cell Cycle
SPECIFICITY:
This antibody detects endogenous level of 14-3-3 zeta only when phosphorylated at serine 58.
Swissprot #:
P63104