ProSci

Paxillin Antibody

Product Code:
 
PSI-79-144
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store antibody at -20˚C for up to one year.
1 / 2
Western blot analysis of lysed extracts from 3T3 cells untreated or treated with serum using Paxillin (Phospho-Tyr118).
2 / 2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin (Phospho-Tyr118).
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Western blot analysis of lysed extracts from 3T3 cells untreated or treated with serum using Paxillin (Phospho-Tyr118).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin (Phospho-Tyr118).

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PSI-79-144-0.1mg0.1mg£488.00
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Further Information

Additional Names:
IKBA, MAD3, NFKBI, PAXI, PXN
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Background References:
  • Tao WA, et al. (2005) Nat Methods. 2 (8): 591-598. Zhang Y, et al. (2005) Mol Cell Proteomics. 4 (9): 1240-1250. Sanders MA, et al. (2005) J Biol Chem. 280 (25): 23516-23522. Rush J, et al. (2004) Nat Biotechnol. 23 (1): 94-101.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Paxillin (Phospho-Tyr118) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 118 (H-V-Y (p) -S-F) derived from Human Paxillin.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5829
NCBI Official Name:
paxillin
NCBI Official Symbol:
PXN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
68 kDa
Protein Accession #:
NP_001074324.1
Protein GI Number:
170932514
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 118.
Swissprot #:
P49023