ProSci

NMDA NR2B Antibody

Product Code:
 
PSI-XPS-1019
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
For long term storage ?80˚C is recommended, but shorter term storage at ?20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
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Western blot of rat hippocampal lysate showing phosphospecific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1472.
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Western blot of rat hippocampal lysate showing phosphospecific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1472.

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PSI-XPS-1019-0.1mL0.1mL£686.00
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Further Information

Application Note:
The labeling of the NR2B protein in Western blots of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide. The antibody also labels bands at 65k and 115k. Applications include Dot Blots (DB) and Western Blots (WB). Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Background:
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer?s, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Tyr1472 on NR2B is phosphorylated and this may lead to the increased expression of the NMDAR at the synapse that plays a role in synaptic plasticity.
Background References:
  • Loftis, J.M. and Janowsky, A., "The N-methyl-D-aspartate receptor subunit NR2B: localization, functional properties, regulation, and clinical implications," Pharmacol. Ther., 97 (2003) 55 - 85.
  • Clayton, D.A., Mesches, M.H., Alvarez, E., Bickford, P.C., and Browning, M.D., "A hippocampal NR2B deficit can mimic age-related changes in long-term potentiation and spatial learning in the Fischer 344 rat," J. Neurosci., 22 (2002) 3628 - 3637.
  • Grosshans, D.R., Clayton, D.A., Coultrap, S.J., and Browning, M.D., "LTP leads to rapid surface expression on NMDA not AMPA receptors in adult rat CA1," Nature Neurosci., 5 (2002) 27 - 33.
  • Snell, L.D., Nunley, K.R., Lickteig, R.L., Browning, M.D., Tabakoff, B., and Hoffman, P.L., "Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion," Mol. Brain Res., 40 (1996) 71 - 78.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
NMDA NR2B (Tyr1472) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphorylated Tyr1472 of rat NMDA NR2B.
Modifications:
Phospho-Specific
NCBI Gene ID #:
24410
NCBI Official Name:
glutamate receptor, ionotropic, N-methyl D-aspartate 2B
NCBI Official Symbol:
Grin2b
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
180
Protein Accession #:
Q00960
Protein GI Number:
548372
Purification:
Affinity Purified
Research Area:
Neuroscience ,Phospho-Specific
SPECIFICITY:
NMDA antibody is specific for ~180 kD NMDAR NR2B phosphorylated at Tyr1472.
Swissprot #:
Q00960
User NOte:
Optimal dilutions for each application to be determined by the researcher.