Synapsin Antibody

Product Code:

PSI-XPS-1026

Supplier:

ProSci

Host Type:

Rabbit

Antibody Clonality:

Polyclonal

Regulatory Status:

RUO

Target Species:

Rat

Application:

Western Blot (WB)

Storage:

For long term storage ?80˚C is recommended, but shorter term storage at ?20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

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Code	Size	Price

PSI-XPS-1026-0.1mL

0.1mL

£686.00

Quantity:

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Further Information

Application Note:

Immunolabeling of the 78k Synapsin I doublet in a Western blot of rat brain homogenate is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Background:

Synapsin I plays a key role in synaptic plasticity in brain. This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation. Thus synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation. Serine 9 is the site on synapsin I that is phosphorylated by cAMP-dependent protein kinase and by calcium calmodulin kinase I. Phosphorylation of this site is thought to regulate synaptic vesicle function.

Background References:

Kao, H.T., Song, H.J., Porton, B., Ming, G.L., Hoh, J., Abraham, M., Czernik, A.J., Pieribone, V.A., Poo, M.M., and Greengard, P., "A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth," Nature Neurosci., 5 (2002) 431 - 437.
Jovanovic, J.N., Sihra, T.S., Nairn, A.C., Hemmings, H.C., Jr., Greengard, P., and Czernik, A.J., "Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals," J. Neurosci., 21 (2001) 7944 - 7953.
Menegon, A., Dunlap, D.D., Castano, F., Benfenati, F., Czernik, A.J., Greengard, P., and Valtorta, F., "Use of phosphosynapsin I-specific antibodies for image analysis of signal transduction in single nerve terminals," J. Cell Sci., 113 (2000) 3573 - 3582.
Hosaka, M, Hammer, R.E., Sudhof, T.C., "A phospho-switch controls the dynamic association of synapsins with synaptic vesicles," Neuron, 24 (1999) 377-87.

Concentration:

batch dependent

Conjugate:

Unconjugated

Immunogen:

Synapsin (Ser603) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphorylated Ser609 of Rat Synapsin I.

Modifications:

Phospho-Specific

NCBI Gene ID #:

281510

NCBI Official Name:

synapsin I

NCBI Official Symbol:

SYN1

NCBI Organism:

Bos taurus

Physical State:

Liquid

PREDICTED MOLECULAR WEIGHT:

Protein Accession #:

P17599

Protein GI Number:

1351166

Purification:

Affinity Purified

Research Area:

Neuroscience ,Phospho-Specific

SPECIFICITY:

Synapsin antibody is specific for Synapsin I phosphorylated at Ser603.

Swissprot #:

P17599

User NOte:

Optimal dilutions for each application to be determined by the researcher.

Synapsin Antibody

Further Information

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