ProSci

Estrogen Receptor-a Antibody

Product Code:
 
PSI-79-306
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store antibody at -20˚C for up to one year.
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Western blot analysis of lysed extracts from MCF cells using Estrogen Receptor-α (Ab-167).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (Ab-167).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-α (Ab-167).
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Western blot analysis of lysed extracts from MCF cells using Estrogen Receptor-α (Ab-167).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (Ab-167).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-α (Ab-167).

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PSI-79-306-0.1mg0.1mg£481.00
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Further Information

Additional Names:
ER, ESR, Era, ESRA, ESTRR, NR3A1, LIMK, ER, ESR1, ESTR, ESTRA
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
Background References:
  • H Yamaguchi, J Zhu, T Yu, et al. (2007) Serum-free mouse embryo cells generate a self-sustaining feedback loop for an astrocyte marker protein and respond to cytokines and bisphenol A in accordance with the subtle difference in their differentiation state. Cell Biology International, 31 (6): 638-644.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Estrogen Receptor-a (Ab-167) antibody was raised against a peptide sequence around aa.165~169 (L-A-S-T-N) derived from Human Estrogen Receptor-α.
NCBI Gene ID #:
2099
NCBI Official Name:
estrogen receptor 1
NCBI Official Symbol:
ESR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66 kDa
Protein Accession #:
NP_000116.2
Protein GI Number:
62821794
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total Estrogen Receptor-α protein.
Swissprot #:
P03372