ProSci

Nanog Antibody

Product Code:
 
PSI-79-644
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store antibody at -20˚C for up to one year.
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Western blot analysis of lysed extracts from human umbilical cord mesenchymal stem cell using Nano g Antibody.
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Western blot analysis of lysed extracts from human umbilical cord mesenchymal stem cell using Nano g Antibody.

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PSI-79-644-0.1mg0.1mg£481.00
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Further Information

Additional Names:
FLK1, VEGFR2, Homeobox transcription factor Nanog
Application Note:
Western Blot: 1:1000
Background:
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes By similarity. Acts as a transcriptional activator or repressor By similarity. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' By similarity. When overexpressed, promotes cells to enter into S phase and proliferation
Background References:
  • Do HJ, et al. Biochem Biophys Res Commun. 2007 Feb 16;353 (3): 770-5. Boyer LA, et al. Cell. 2005 Sep 23;122 (6): 947-56. Freberg CT, et al. Mol Biol Cell. 2007 May;18 (5): 1543-53. Chambers I, et al. Cell. 2003 May 30;113 (5): 643-55.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Nanog antibody was raised against a peptide sequence around aa.137~141 (K-Q-V-K-T) derived from Nano g.
NCBI Gene ID #:
79923
NCBI Official Name:
Nanog homeobox
NCBI Official Symbol:
NANOG
NCBI Organism:
This antibody was raised against a peptide sequence around aa.137~141 (K-Q-V-K-T) derived from Nano g
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
NP_079141.2
Protein GI Number:
153945816
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Stem Cell
SPECIFICITY:
This antibody detects endogenous level of total Nanog protein.
Swissprot #:
Q9H9S0