ProSci

SNAP25 Antibody

Product Code:
 
PSI-45-149
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-149 (0.03ug/ml) staining of Human Cerebellum (A), Mouse (B) and (0.05ug/ml) Rat Brain (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-149 (0.03ug/ml) staining of Human Cerebellum (A), Mouse (B) and (0.05ug/ml) Rat Brain (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-45-149-0.1mg0.1mg£626.00
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Further Information

Additional Names:
SNAP25, synaptosomal-associated protein, 25kDa , HGNC:11132, FLJ23079, SNAP, SNAP-25, bA416N4.2, dJ1068F16.2 , synaptosomal-associated protein 25, RIC-4, RIC4, SEC9, resistance to inhibitors of cholinesterase 4 homolog, synaptosomal-associated protein 25
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in Human Cerebellum and in Mouse and Rat Brain lysates (calculated MW of 23.3kDa according to Human NP_003072.2, Mouse NP_035558.1 and Rat NP_001257504.1). Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Cerebellum shows strong axon staining, particularly in the granular region. Recommended concentration: 1-2ug/ml.
Background References:
  • Voeller KK. Attention-deficit hyperactivity disorder (ADHD). J Child Neurol. 2004 Oct;19(10):798-814. Review.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-DEANQRATKMLGSG
NCBI Gene ID #:
6616
NCBI Official Name:
synaptosomal-associated protein, 25kDa
NCBI Official Symbol:
SNAP25
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003072.2, NP_570824.1
Protein GI Number:
18765733
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis ,Neuroscience
SPECIFICITY:
This antibody is expected to recognise both reported isoforms NP_003072.2 (SNAP25A) and NP_570824.1 (SNAP25B). Reported variants represent identical protein: NP_001309831.1, NP_003072.2 Reported variants represent identical protein: NP_001309834.1, NP_001309832.1, NP_001309833.1, NP_001309839.1, NP_001309836.1, NP_570824.1, NP_001309838.1, NP_001309835.1, NP_001309837.1
Swissprot #:
P60880