ProSci

sIL-2Ra Monoclonal Antibody

Product Code:
 
PSI-38-230
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Clonality:
 
Monoclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
 
sIL-2Ra lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-
1 / 1
This antibody stained frozen human tonsil. The recommended concentration is 5.0 ug/ml at room temperature. A labeled polymer detection system was used with DAB chromogen. Optimal concentrations and conditions may vary.
❮❯

This antibody stained frozen human tonsil. The recommended concentration is 5.0 ug/ml at room temperature. A labeled polymer detection system was used with DAB chromogen. Optimal concentrations and conditions may vary.

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CodeSizePrice
PSI-38-230-0.5mg0.5mg£488.00
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This product comes from: United States.
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Further Information

Additional Names:
Interleukin-2 receptor subunit alpha, TAC antigen, IL-2 receptor subunit alpha, CD25, IL2R, TCGFR, IDDM10
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hsIL-2Rα (10.0 μg/mL), a concentration of 1.0-1.5 μg/mL of this antibody is required, in the presence of 0.5 ng/mL recombinant hIL-2.

ELISA:

In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human sIL-2Rα when used in conjunction with compatible secondary reagents.

Western Blot:
To detect hsIL-2Rα by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsIL-2Rα is 2.0-4.0 ng/lane, under reducing or non-reducing conditions
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
sIL-2Ra antibody is produced in mice using highly pure (>98%) recombinant human soluble IL-2 Receptor alpha as the immunizing antigen.
NCBI Gene ID #:
3559
NCBI Official Name:
interleukin 2 receptor, alpha
NCBI Official Symbol:
IL2RA
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P01589
Protein GI Number:
124317
Purification:
This IgG2aK antibody was purified from cell culture by Protein G affinity chromatography.
Swissprot #:
P01589
User NOte:
Centrifuge vial prior to opening.